碳酸酐酶在四氧化三铁上固定化及其性能表征研究

An Investigation of the Immobilization of Carbonic Anhydrase on Fe3O4 and Its Performance Characterization

摘要:

以四氧化三铁为载体，利用共价结合和交联法相结合的方法制备固定化碳酸酐酶（CA），通过单因素试验，得出CA最佳固定化条件，并对固定化酶进行性能表征。结果表明，在最优的固定化条件下，固定化酶的酶活回收率可达到66.90%，且其pH值稳定性、操作稳定性、热稳定性和贮藏稳定性明显高于同等条件下的游离酶，该固定化酶经5次催化水解底物后仍能保持62.58%的相对酶活。

Abstract:

Abstract: Carbonic anhydrase (CA) was immobilized on Fe3O4 by means of the covalent bonding and crosslinking method. The conditions of single factor, such as pH, temperature, time, shaker speed, enzyme dose, glutaraldehyde dose, and glutaraldehyde time, were studied. And then the optimum conditions and properties of immobilization CA were obtained. The results showed that the rate of enzyme activity recovery of immobilized enzyme is 66.90% under the optimum conditions. And the pH stability, operational stability, thermal stability and storage stability of immobilized CA are significantly higher than that of the free CA. Particularly, the immobilized CA could keep 62.58% relative enzyme activity after 5 times of catalytic hydrolysis with the substrate. enzyme activity after 5 times of catalytic hydrolysis with the substrate.