利用响应面优化固态发酵生产毛叶山桐子脱脂果渣发酵饲料

Optimization of response surface for fermented feed in Idesia polycarpa residues through solid-state fermentation

摘要:

文章利用响应面法对毛叶山桐子发酵饲料的发酵条件进行了优化。实验菌种采用了产朊假丝酵母，实验材料为毛叶山桐子的脱脂果渣。首先利用单因素实验对五个影响发酵的指标进行了研究，然后在单因素的基础上利用Plackett-Burman实验设计筛选出其中显著的三个因子并利用Box-Behnken响应面设计对其进行优化。优化后的条件为发酵温度30℃，料液比1：1.2，接种量10.5%，硝酸铵添加量8.8%，发酵时间4天。经优化条件发酵后，毛叶山桐子脱脂果渣发酵饲料的粗蛋白由发酵前的8.49%提升到19.13%，脱脂果渣的营养组成变得更加合理。表明毛叶山桐子脱脂果渣作为发酵饲料应用于养殖产业具有较高的价值和潜力。

Abstract:

In this study, response surface methodology (RSM) was performed the optimization of the fermentation of Idesia polycarpa residues, and Candida utilis was selected for solid-state fermentation. Five influences of the fermentation were studied by one-factor tests, and the Plackett-Burman experiment subsequent confirmed three main influences for Box-Behnken RSM design. Optimized conditions of the fermentation as follow: residues with ratio of solid to liquid 1:1.2, NH4NO3 additive amount 8.8%, and Candida utilis inoculation 10.5% fermented in 30℃ for 4 days. Fermentation results showed that, the crude protein content of fermented feed increased from 8.49% to 19.13%, and the nutrition ratio of products became more reasonable. This study suggests that Idesia polycarpa residues have a real potential on the modern farming by the solid-state fermentation.