长非编码RNA LINC00941在结直肠癌中的表达及对细胞增殖的影响研究

The expression of long non-coding RNA LINC00941 in colorectal cancer and effect on cell proliferation

摘要:

为了研究与细胞分化相关的长非编码RNA(long non-coding RNA, lncRNA) LINC00941在肿瘤发生发展中的作用，通过实时荧光定量PCR技术检测LINC00941在6种不同类型的人类癌细胞和正常胚胎肾细胞HEK-293细胞中的表达水平，结果表明，LINC00941在结直肠癌细胞HCT116和HCT116 p53-/-、肺癌细胞A549和NCI-H1299、黑素瘤细胞Stilling中均有较高的表达水平，在结直肠癌细胞中表达水平最高. 以结直肠癌患者肿瘤组织和癌旁正常组织为材料，实时荧光定量PCR检测LINC00941的表达水平发现，肿瘤组织中LINC00941 RNA的表达水平显著高于癌旁组织. 通过shRNA(short hairpin RNA)干扰技术降低HCT116细胞中的LINC00941 RNA水平，导致细胞增殖速度下降，说明LINC00941与结直肠癌的发生发展相关.

Abstract:

To explore the function of a cell differentiation-associated long non-coding RNA (lncRNA) LINC00941 in cancer progression, we detected the expression of LINC00941 in 6 types of human cancer cell lines and HEK-293 cell line by real-time quantitative PCR. LINC00941 is highly expressed in colorectal carcinoma cell lines HCT116 and HCT116 p53-/-, lung carcinoma cell lines A549 and NCI-H1299, and melanoma cell line Stilling, especially in colorectal carcinoma cell lines. By detecting the RNA levels of LINC00941 in the tumor and normal tissues of colorectal cancer patients, we found that the expression levels were significantly increased in tumor compared with paired normal tissues. By shRNA(short hairpin RNA) interfering, we found LINC00941-knockdown HCT116 cells had lower proliferation rate compared with negative control cells, suggesting the correlation of LINC00941 and colorectal cancer progression.