极危物种——南川木波罗遗传多样性ISSR分析

ISSR analysis of the genetic diversity of Artocarpus nanchuanensis， a critically endangered species, in Nanchuan District, Chongqing

摘要:

本研究利用ISSR-PCR分子标记技术分析研究了重庆市七个地区野生生长的46株南川木波罗（Artocarpus nanchuanensis）样品的遗传多样性.利用筛选出的12条UBC引物共扩增出了117条DNA片段，其中多态性片段为113条，多态条带百分率为96.58%.NTSYS~pc2.1软件分析表明：七个地区南川木波罗之间的遗传相似性介于0.062~0.989之间，并用UPGMA法构建了系统树.结果表明该市野生生长的南川木波罗既有相同的遗传背景，又存在较明显的差异.46份材料划分为4类，聚类基本符合地理来源相近的材料聚为一类，呈现出一定的地域性分布规律.

Abstract:

Genetic diversity of 46 strainswild Artocarpus nanchuanensis from seven areas of Chongqing was preliminary analyzed using inter-simple sequence repeat (ISSR) PCR molecular marker technology. The selected 12 pairs of UBC primers were used in this research, and a total of 117 DNA fragments were obtained, of which 113 were polymorphic fragments, and the percentage of polymorphic bands was 96.58%. The analysis by NTSYS pc2.1 software showed that the genetic similarity among Artocarpus nanchuanensis from the seven areas was ranged from 0.062~0.989, and the dendrogram was constructed through UPGMA method. The results revealed that these Artocarpus heterophyllus Lam. have a similar genetic background with obvious differences. These Artocarpus heterophyllus Lam. were obviously divided into four groups, and the cluster is in line with the similar geographical origin of materials, showing a certain regional distribution.