高氨氮对廖叶眼子菜光合及抗氧化系统影响的分析

Effects of high ammonium nitrogen on photosystem and antioxidant system of Potamogeton polygonifolius

摘要:

在不同质量浓度氨氮条件下，对廖叶眼子菜(Potamogeton polygonifolius)体内氧化物质含量、抗氧化酶活性和叶绿素荧光变化进行了研究.实验结果显示：高质量浓度氨氮（10 mg/L）下廖叶眼子菜的光合作用能力所受影响不大.但是高质量浓度氨氮（5~10mg/L）会提高廖叶眼子菜体内氧化物质含量，植物自身防御机制进而会在一定时间内促使体内抗氧化酶活力及次生物质含量的上升，其中氨氮质量浓度为10 mg/L时抗氧化酶及次生物质反应强烈.结果表明氧化能力的上升有助于缓解氨氮诱导的氧化胁迫及增强植物对氨氮的耐受性.总体而言，较高的氨氮浓度会对廖叶眼子菜具有一定的影响，在使用该植物修复水体时需要考虑氨氮的浓度水平及其影响.

Abstract:

Changes in concentrations of oxidative material, antioxidase activities and chlorophyll fluorescence were investigated in Potamogeton polygonifolius exposed to different concentrations of ammonium nitrogen. The results show that 10mg/L of ammonium nitrogen had a little effect on photosynthetic capacity of P. polygonifolius. However, the 5 mg/L to 10 mg/L concentration of ammonium nitrogen could increase oxygen radicals and enhance the defensive capacity by stimulating the antioxidant enzymes activity and increasing content of secondary metabolites. 10 mg/L concentration of ammonium nitrogen had significant effect on the activity of antioxidant enzymes and content of secondary metabolites. The increased antioxidant capacity may alleviate ammonium induced oxidative stress, and thus enhance the endurance of plants to cope with ammonium stress. Overall, higher concentration of ammonium nitrogen had some effect on P. polygonifolius and consequently, the concentration and impact of ammonium nitrogen should be considered when using P. polygonifolius was chosen as candidate plants for the to remediate water ecosystem.