中华蜜蜂幼虫肠道响应球囊菌胁迫的可变剪切基因分析

Analysis of the alternatively spliced genes in Apis cerana cerana larval gut under the Ascosphaera apis stress

摘要:

基于前期已获得的中华蜜蜂（简称中蜂）幼虫肠道转录组数据，利用TopHat2软件在正常（AcCK）及球囊菌胁迫的中蜂幼虫肠道样品（AcT1、AcT2、AcT3）中共鉴定出发生于9124个基因的57327个可变剪切事件，其中以基因间（17.68%）、可变3′端剪切（15.32%）、外显子跨越（14.12%）和可变5′端剪切（12.81%）类型为主.Venn分析结果显示4个肠道样品的共有可变剪切基因数为8111个，特有可变剪切基因数分别为272、189和385个.GO分类结果显示共有可变剪切基因涉及47个条目，AcT1、AcT2、AcT3的特有可变剪切基因分别富集于24、20和34个条目.KEGG代谢通路富集分析结果显示，共有可变剪切基因富集在327个代谢通路，基因富集数最多的是RNA转运、内质网蛋白加工及核糖体；AcT1、AcT2、AcT3的特有可变剪切基因分别富集在22、46和83个代谢通路.结果揭示了可变剪切基因在宿主的胁迫响应过程中的重要作用.

Abstract:

Based on previous obtained transcriptome data of the larval gut of Apis cerana cerana, a total of 57327 alternative splicing events occurred in 9124 genes were identified, in normal guts (AcCK) and Ascosphaera apis infected guts (AcT1, AcT2 and AcT3). among them, intergenic(17.68%), p3 splice (15.32%), exon span (ES) (14.12%) and p5 splice (12.81%) were main kinds of alternative splicing. Venn analysis showed 8111 alternatively spliced genes were shared in these four samples, and the number of unique alternatively spliced genes in AcT1, AcT2 and AcT3 are 272, 189 and 385, respectively. GO categorization demonstrated the shared alternative spliced genes were engaged in 47 GO terms, and the unique alternatively spliced genes in AcT1, AcT2 and AcT3 were related to 24, 20 and 34 GO terms. KEGG pathway enrichment analysis displayed the shared alternatively spliced genes were enriched in 327 pathways, and the largest ones were RNA transport, protein processing in endoplasmic reticulum and ribosome; the unique alternatively spliced genes of AcT1, AcT2 and AcT3 were enriched in 22, 46 and 83 pathways, respectively.