高通量抗体芯片研究AILD血清炎性因子表达特征

Detection of serum cytokines of autoimmune liver disease patients by high throughput cytokine antibody microarray

摘要:

利用微阵列抗体芯片技术检测53例自身免疫性肝病（AILD）患者血清中20种辅助性T细胞相关炎性因子的表达水平，结合肝功能临床生化数据，分析炎性因子的表达特征和差异性.高通量抗体芯片检测结果显示：AIH-PBC OS患者血清中IL4和TGFβ1表达量水平显著高于PBC组（P<0.05），AI-PBC OS患者血清中IL4表达量水平显著高于健康对照组（P<0.05），PBC患者血清中IL-4和TGFβ1表达量水平显著低于健康对照组（P<0.05）.PBC患者血清中TGFβ1表达量与ALT（r=0.6301,P=0.0135）、AST（r=0.7443, P=0.0030）呈显著负相关.结果表明：IL4和TGFβ1在AIH-PBC OS患者和PBC患者血清中表达量差异（P<0.05）和TGFβ1表达量与AILD患者肝功能指标间的相关性为筛选具有临床检验、诊断、治疗和预后监测意义的AILD相关细胞因子奠定了基础.

Abstract:

The antibody microarray technique was used to detect the expression of 20 kinds of helper T cellassociated inflammatory cytokines in serum of 53 patients suffered with autoimmune liver disease (AILD). Combining with the clinical biochemical data of liver function to analyze the expression characteristics and differences in inflammatory factors in serum of patients with autoimmune liver disease (AILD). The results showed that the levels of IL4 and TGFβ1 in serum of patients with AIH-PBC OS were significantly higher than those of PBC (P<0.05), while the levels of IL-4 in serum of patients with AIH-PBC OS were significantly higher than that of healthy controls (P<0.05). The levels of IL-4 and TGFβ1 in serum of PBC patients were significantly lower than those of healthy controls (P<0.05).The level of TGFβ1 in serum of PBC patients was negatively correlated with ALT (r=-0.6301, P=0.0135) and AST (r=-0.7443, P=0.0030). Conclusion: the expression levels of IL-4 and TGFβ1 in the blood serum of AIH-PBC OS, PBC patients and the expression level of TGFβ1 was of favorable correlation with the liver function indexes of AILD patients，which indicates it is potential application in AILD screening, diagnosis, and prognosis.