水稻STN7激酶突变体的光合生理特性分析

Analysis of photosynthetic physiological characteristics of loss-of-function of STN7 in rice ( Oyrza sativa)

摘要:

本实验旨在研究野生型与stn7突变体在光合性能上的差异.实验包括检测种子萌发状况、用脉冲幅度调制成像荧光计IMAGMINI测量叶绿素含量、用SPAD502 叶绿素仪测量SPAD值以及用LI6400XT便携式光合仪测量叶绿素荧光动力学参数并用photosynthesis软件拟合光响应曲线及相关参数.结果显示,WT和stn7植株的种子萌发情况和SPAD值几乎无差异,叶绿素荧光动力学参数显示WT的光合性能比stn7植株高,叶绿素a/b和光响应曲线的结果得出WT利用弱光的能力较强,光响应曲线结果还显示高光强下,stn7植株光合速率较高.本文研究发现STN7激酶在调节水稻的光合作用中发挥着重要作用.

Abstract:

In this experiment, it was designed to investigate the differences in photosynthetic performance between wildtype (WT) and stn7 mutant. The seed germination rate of two rice was detected. Besides, chlorophyll content was measured by pulse amplitude modulation imaging fluorometer IMAGMINI, SPAD502 chlorophyll meter was used to measured the SPAD value of plants in the seedling stage, and the light response curve of the plants at jointingbooting stage under natural growth was measured by the LI6400XT portable photosynthesis system. The results showed that there was almost no difference in seed germination and SPAD value between WT and stn7. The result of chlorophyll fluorescence parameters indicates that the photosynthetic performance of WT is better than stn7. The result of chlorophyll a/b value and light response curve reveals the ability to use low light in WT is stronger. And the result of the light response curve shows that the photosynthetic rate of stn7 is higher than WT in high light. These results attest that the STN7 kinase is important for the photosynthesis of rice