桑树桑黄与杨树桑黄的分子辨别

Molecular identification of Sanghuangporus species S. sanghuang and S.vaninii

摘要:

为准确鉴别桑黄（Sanghuangporus）近缘种桑树桑黄（S.sanghuang）与杨树桑黄（S.vaninii）、暴马桑黄（S.baumii），本研究基于核糖体基因rDNA内转录间隔区（internal transcribed spacer，ITS）序列分析技术，对桑黄真菌进行了系统发育分析与近缘种的分子辨别研究.结果表明，在NJ系统发育树上，23个桑树桑黄、11个杨树桑黄和6个暴马桑黄菌株各自以很高的Bootstrap支持率聚为了三个独立的分支，种间差异明显.依据桑树桑黄和杨树桑黄的rDNA ITS序列差异，设计两对引物Sv_U1/Sv_L和Sv_U2/Sv_L，均可特异性地扩增杨树桑黄478 bp和651 bp的ITS片段，而不扩增桑树桑黄的ITS片段，因而可用于两个桑黄近缘种的快速分子辨别.本研究为探讨桑黄孔菌属真菌的系统发育关系提供了参考，并为桑黄种间的准确辨别提供了一种有效的分子辅助手段.

Abstract:

For accurate identification of three relative Sanghuangporus species including S. sanghuang, S.vaninii and S. baumii, the phylogenetic analysis and molecular recognition of relative Sanghuangporus species were performed based on the internal transcribed spacer (ITS) sequence of the ribosome rDNA. NJ phylogenetic tree indicated that 23 S.sanghuang strains, 11 S. vaninii strains and 6 S. baumii strains formed three independent clades with a high bootstrap support rate, and the obvious differences among the three relative species were presented. Based on the difference in rDNA ITS sequence alignment of S.sanghuang and S.vaninii, two pairs of primers, Sv_U1/Sv_L and Sv_U2/Sv_L, were designed, which can specifically amplify two ITS fragments of 478 bp and 651 bp only from S.vaninii. but not from S. sanghuang, indicating that this method can be effectively used for the molecular recognition of the two relative Sanghuangporus species. This study provides the evidence for the phylogenetic relationship of Sanghuangporus genus, and an effective molecular assisted tool for the precise recognition of relative Sanghuangporus species.