麻疯树根高效再生体系的建立及不定芽起源研究

The study of plant regeneration from root explants of Jatropha curcas and origin of adventitious shoots

摘要:

为了克服现有麻疯树快繁技术的不足,分别以麻疯树胚根和子叶外植体诱导的不定根为外植体诱导不定芽.麻疯树子叶不定根诱导的最佳培养方式为:MS+5 mg/L IBA上培养4的,然后转至MS0培养基上生根.诱导生根率和生根数分别达到93.33%和6.54个/外植体.子叶不定根诱导不定芽的最佳培养基是MS+3.0 mg/L 6-BA+3 mg/L IAA,诱导率达到83.33%.麻疯树胚根的最佳不定芽诱导培养基为MS+0.2 mg/L 6-BA+1.0 mg/L IBA,分化率达67.80%,平均出芽数为3.25个/外植体.对麻疯树胚根再生的各阶段进行了石蜡切片观察,明确了不定芽起源于根的薄壁细胞层.与现有技术相比,本方法具有周期短、再生效率高的特点.

Abstract:

In order to overcome the shortcomings of the existing propagation technology of Jatropha curcas, adventitious buds were induced from the adventitious root explants of cotyledon and radicle explants respectively. The optimal way to get adventitious root from cotyledons of Jatropha curcas L. was that it would be precultured on the MS medium containing 5 mg/L IBA for 4 days, then transferred to MS0 medium for rooting. The rooting inducing rate and adventitious root number reached 93.33% and 6.54/explant, respectively. The optimal medium for adventitious buds inducing was MS+3.0 mg/L 6-BA+3 mg/L IAA. The induction rate could reach 83.33%. The optimal adventitious bud induction medium from the radicle explants of Jatropha curcas L. was MS+0.2 mg/L 6-BA+1.0 mg/L IBA. The differentiation rate was 67.80%, and the average number of sprouts was 3.25 per explant. Paraffin sections were observed at each stage of bud regeneration and it seems that adventitious buds originate from parenchyma of roots. Compared with the prior methodology, the method presented here has the characteristics of a shorter cycle and higher regeneration efficiency.