簸箕柳AP2/ERF家族基因的全基因组分析

Genome-wide analysis of the AP2/ERF family genes in Salix suchowensis

摘要:

为了研究簸箕柳AP2/ERF转录因子家族的成员和分类，及后续深入探究其在簸箕柳生长发育过程中的功能，本文基于簸箕柳基因组数据，利用生物信息学技术和方法，对簸箕柳的AP2/ERF家族基因进行鉴定、分类及染色体定位，并对其理化性质、基因结构、保守结构域、表达模式等进行分析.结果表明，簸箕柳含有178个AP2/ERF家族基因，并根据AP2/ERF保守域的数量与拟南芥AP2/ERF家族基因的进化关系将其划分成AP2、ERF、RAV和Soloist亚家族4类.此外组织表达模式分析结果显示，簸箕柳AP2/ERF家族基因的表达具有明显的组织特异性，且在leaf、root、shoot、flower中表达较强，在其他组织中表达较弱.

Abstract:

In order to study the members and classification of the AP2/ERF transcription factor family of Salix suchowensis, and to further explore its function in the growth and development, the AP2/ERF family genes were identified, classified, chromosomed, and their physical and chemical properties, gene structure, conserved domains, and expression patterns were also analyzed using bioinformatics techniques and methods. The results showed that Salix suchowensis contains 178 AP2/ERF genes, which were classified into four subfamilies: AP2, ERF, RAV, and Soloist according to the evolutionary relationship between the AP2/ERF conserved domain and the AP2/ERF genes of Arabidopsis thaliana. In addition, the results of tissue expression pattern analysis showed that the AP2/ERF family genes had obvious tissue specificity, it was strongly expressed in leaf, root, shoot, and flower, and weakly expressed in other tissues.