UPLC测定纤毛婆婆纳主要环烯醚萜苷成分的含量

Study on the content determination of main iridoid glycosides in Veronica ciliata Fisch by UPLC

摘要:

建立超高效液相色谱法（UPLC）同时测定纤毛婆婆纳中梓醇、梓苷、胡黄连苷Ⅱ和6-O-藜芦酰梓苷4种主要环烯醚萜苷类成分含量.采用Waters ACOUITY T3色谱柱（100 mm×2.1 mm，1.8 μm）；柱温为40 ℃；流动相为乙腈-水（0.1%甲酸+2.5 mmol甲酸铵）；流速为0.4 mL/min；检测波长为260 nm.结果表明梓醇、梓苷、胡黄连苷Ⅱ和6-O-藜芦酰梓苷4种主要环烯醚萜苷类成分含量.结果表明梓醇、梓苷、胡黄连苷Ⅱ和6-O-藜芦酰梓苷分别在1.562～50 μg/mL（R2＝0.9987）、1.562～50 μg/mL（R2＝0.9989）、4.689～143 μg/mL（R2＝0.9996）、1.562～50 μg/mL（R2＝0.9998）范围内呈现良好的线性关系，平均回收率分别为100.21%（RSD＝1.3%）、99.43%（RSD＝1.8%）、100.6%（RSD＝2.5%）、102.7%（RSD＝0.65%）.该方法简便、灵敏、经济、可靠，可用于纤毛婆婆纳的质量控制和评价.

Abstract:

A UPLC method has been developed in the current investigation for simultaneous determination of four main iridoid glycosides, which were verproside, catalposide, amphicoside and 6-O-veratroylcatalposide in Veronica ciliata Fisch. The separation was performed at 40 ℃ on Waters ACOUITY T3 column (100 mm×2.1 mm, 1.8 μm); the mobile phase was acetonitrile-water(0.1% formic acid+2.5 mmol ammoniumformate); the flow rate was 0.4 mL/min and the detection wavelength was 260 nm. The results showed that the calibration curves were the good linear relationships in the range of 1.562～50 μg/mL (R2＝0.9987) for verproside, 1.562～50 μg/mL (R2＝0.9989) for catalposide, 4.689～143 μg/mL (R2＝0.9996) for amphicoside, 1.562～50 μg/mL (R2＝0.9998) for 6-O-veratroylcatalposide, the average recovery rate were 100.21%(RSD=1.3%), 99.43% (RSD=1.8%), 100.6% (RSD=2.5%), 102.7% (RSD=0.65%),respectively. The method is simple, sensitive, economical and reliable, and can be used for quality control and evaluation of Veronica ciliata Fisch.