虾青素抑制H2O2诱导的HeLa细胞凋亡

Protective role of astaxanthin against H2O2-induced apoptosis in HeLa cells

摘要:

为了阐明虾青素的抗氧化作用与细胞凋亡的关系，探究虾青素预处理对H2O2诱导HeLa细胞氧化应激的影响. 通过CCK-8、活性氧探针染色、流式细胞术、蛋白质免疫印迹、实时荧光定量pcr等，分别检测细胞存活率和活性氧的积累、细胞凋亡、蛋白含量、基因相对表达量改变. 结果表明虾青素预处理组细胞活力较对照组提高了29.54%以上且其可以将H2O2诱导的活性氧降低至对照水平，同时提高Nrf2蛋白表达量3倍之多，过氧化氢酶基因相对表达量1.5倍. 说明虾青素可以有效缓解H2O2诱导的HeLa细胞氧化应激，从而抑制细胞凋亡.

Abstract:

In order to clarify the relationship between Astaxanaxin's antioxidant effect and cell apoptosis, cell survival rate, accumulation of reactive oxygen species, cell apoptosis, protein content and relative gene expression were detected by CCK-8, reactive oxygen probe staining, flow cytometry, western blotting, and realtime quantitative fluorescence PCR to explore the effect of astaxanthin pretreatment on oxidative stress induced by H2O2 in HeLa cells. The results showed that the cell activity of astaxanaxin pretreatment group was 29.54% higher than that of the control group, and it could reduce H2O2-induced reactive oxygen species to the control levels, meanwhile, it can also increase the Nrf2 protein expression by 3 times, and increase the relative expression of the catalase gene by 1.5 times. The results indicated that astaxanthin could effectively alleviate the oxidative stress of HeLa cells induced by H2O2, thereby inhibiting apoptosis.