甘蓝型油菜BnHY5基因的表达模式及非生物胁迫响应分析

Expression pattern and abiotic stress response analysis of BnHY5 in Brassica napus L.

摘要:

为探究甘蓝型油菜中HY5的表达情况，首先通过甘蓝型油菜基因组数据分析预测HY5及其同源基因HYH（HY5-Homologous），克隆获得了四个HY5（命名为BnHY5）以及三个HYH（命名为BnHYH）重复基因全长cDNA序列.其次，对BnHY5进行了蛋白结构预测、理化性质分析，研究结果表明：BnHY5蛋白为不稳定、亲水性蛋白，主要结构域为亮氨酸拉链. 最后，探究了甘蓝型油菜中该基因的表达情况，用荧光定量PCR分析了BnHY5重复基因表达模式和响应非生物胁迫的情况，BnHY5四个基因中，BnHY5-2的表达水平最高，BnHY5-1、BnHY5-2不存在组织表达差异且表达水平高于BnHY5-3、BnHY5-4. BnHY5基因不仅响应高温、低温、镉、高盐非生物胁迫，而且参与ABA和GA3激素信号响应，说明BnHY5在逆境胁迫中有重要作用.此外BnHY5重复基因在非生物胁迫下的表达模式发生改变，BnHY5四个基因的表达占比与不同类型胁迫处理相关，存在表达差异.

Abstract:

In order to explore the expression of HY5 in Brassica napus L., firstly, HY5 and its homologous gene HYH (HY5-Homologous) were predicted by genomic data analysis, and the full-length cDNA sequences of four duplication genes of HY5 (named BnHY5) were cloned. As well as three duplication HYH (named BnHYH) were also cloned. Then, we predicted and analyzed the protein structure of BnHY5 and its chemical properties. The results showed that BnHY5 protein is an unstable and hydrophilic protein, and the main domain is leucine zipper. Finally, BnHY5 duplication gene expression pattern was analyzed by real-time PCR with normal growth conditions and abiotic stress. The results showed that among 4 BnHY5 genes, BnHY5-2 has the highest expression level. BnHY5-1 and BnHY5-2 have no tissue expression differences and the expression level is higher than BnHY5-3, BnHY5-4. BnHY5 gene not only responds to high temperature, low temperature, cadmium, high salt abiotic stress but also participates in ABA and GA3 hormone signal responses, indicating that BnHY5 plays an important role in abiotic stress. In addition, compared with the expression of BnHY5 duplications in normal growth rape, the expression level of BnHY5 duplications under different types of stress treatment changed. The expression ratio of the four genes of BnHY5 has a relationship to different types of stress treatment.