ARID1A对结肠癌细胞迁移的调控作用

Regulation of ARID1A on the migration of colon cancer cells

摘要:

为研究ARID1A对结肠癌细胞迁移的影响，并进一步分析ARID1A调控细胞迁移的机制，本文通过在结肠癌细胞系HCT116中过表达和干扰ARID1A基因，观察细胞迁移率的变化，通过比较HCT116与正常组织中的基因表达数据，筛选出ARID1A共表达基因，进行GO和KEGG分析.结果显示，过表达ARID1A后，细胞迁移率降低，而干扰ARID1A则与之相反.在1212个相关系数大于0.9的ARID1A共表达基因中，仅有4个基因参与细胞增殖，29个基因参与细胞迁移，涉及趋化因子信号通路、细胞因子受体相互作用等多个信号通路.以上结果说明ARID1A抑制细胞迁移，并可能通过多个信号通路调控细胞迁移.

Abstract:

In order to study the effect of ARID1A on the migration of colon cancer cells, and to further analyze the mechanism of ARID1A regulating cell migration, the change of cell migration rate was observed by overexpression and interference of ARID1A gene in HCT116 cell line, and ARID1A co-expressed genes were screened out for GO and KEGG analysis by comparing the gene expression data between HCT116 and normal tissues. The results showed that overexpression of ARID1A reduced cell mobility, while the interference of this gene would lead to the opposite result. Among 1212 ARID1A coexpressed genes with a correlation coefficient greater than 0.9, there are only 4 genes involved in cell proliferation and 29 genes involved in cell migration, which are associated with chemokine signaling pathway, cytokine receptor interaction and other signaling pathways. It is suggested that ARID1A can inhibit cell migration and regulate cell migration through multiple signaling pathways.