蜜蜂球囊菌菌丝和孢子的比较转录组分析

Comparative transcriptome analysis of Ascosphaera apis mycelium and spore

摘要:

为探究实验室条件下纯培养的蜜蜂球囊菌（Ascosphaera apis）菌丝（AaM）和孢子（AaM）的共有基因、特有基因以及差异表达基因（differentially expressed gene, DEG）与真菌的生长、发育、生殖和致病性的相关性，利用RNA-seq技术和生物信息学方法对AaM和AaS进行比较转录组分析，并结合实时荧光定量PCR验证转录组数据的可靠性.分析结果显示AaM和AaS的共有基因为7 362个，涉及44个功能条目和122条通路；二者的特有基因分别为195和278个.AaM vs AaS比较组含有977个上调基因和687个下调基因，分别涉及32和32个功能条目；此外，上述DEG还涉及糖酵解/糖异生、次级代谢物的生物合成等113条通路.基于比较转录组分析发现球囊菌菌丝和孢子的共有基因、特有基因和DEG可能通过调节细胞活动、代谢进程、内吞作用以及MAPK信号通路等关键途径参与球囊菌的生长、发育和生殖过程.

Abstract:

In order to explore the correlation among the common genes, specific genes and differentially expressed genes (DEGs) of mycelium (AaM) and spore (AaS) samples of Ascosphaera apis cultured in lab condition with the fungal growth, development, reproduction and pathogenicity, comparative transcriptome analysis of AaM and AaS was performed using RNA-seq technology and bioinformatic methods, followed by RT-qPCR to confirm the reliability of the sequencing data. The results showed that there were 7,362 genes shared by AaM and AaS, involving 44 functional terms and 122 pathways. The specific genes of AaM and AaS were 195 and 278, respectively. In AaM vs AaS comparison group, 977 up-regulated genes and 687 down-regulated genes were discovered, which were associated with 32 and 32 functional terms, respectively. Additionally, the DEGs mentioned above were related to 113 pathways such as glycolysis/gluconeogenesis and biosynthesis of secondary metabolites. Based on comparative transcriptome analysis, it’s revealed that the common genes, specific genes and DEGs may participate in the growth, development and reproduction process of A.apis mycelium and spore through regulating several pivotal pathways such as cell activity, metabolic process, endocytosis and MAPK signaling pathway.