Napabucasin对结直肠癌细胞增殖及迁移的影响

Effects of Napabucasin on the proliferation and migration of colorectal cancer cells

摘要:

为了探究新型小分子抑制剂Napabucasin对结直肠癌细胞增殖以及迁移的影响. 首先通过分子模拟对接分析了Napabucasin与STAT3蛋白的互作机制. 然后利用克隆形成实验、细胞划痕实验等方法在多种结直肠癌细胞系中证明了Napabucasin能够显著抑制结直肠癌细胞的集落形成能力以及迁移能力. 进而使用Napabucasin与Wnt信号通路激活剂Wnt agonist 1共处理结直肠癌细胞HCT116，结合蛋白质印迹实验发现，Wnt信号通路介导了Napabucasin对结直肠癌细胞的迁移以及增殖的抑制过程. 研究结果显示，Napabucasin能够在体外抑制结直肠癌细胞的增殖能力以及迁移能力，并且Wnt信号通路参与介导了这一抑制过程.

Abstract:

This study aims to explore the effect of the novel small molecule inhibitor Napabucasin on the proliferation and migration of colorectal cancer cells. Firstly, the interaction mechanism between Napabucasin and STAT3 was analyzed by molecular docking. Then, it was proved that Napabucasin can significantly inhibit the colony formation and migration ability of colorectal cancer cells using clone formation experiments and wound healing assay. Napabucasin and Wnt agonist 1 were then used to cotreat the HCT116 cells, combined with Western blot experiments, it was found that the Wnt signaling pathway mediates the inhibition of the migration and proliferation of colorectal cancer cells by Napabucasin. The research results show that Napabucasin can inhibit the proliferation and migration ability of colorectal cancer cells in vitro, and the Wnt signaling pathway is involved in mediating this inhibition process.