欧洲山杨bHLH转录因子家族全基因组分析

Genome-wide analysis of the bHLH transcription factor family in Populus tremula

摘要:

为了研究欧洲山杨中bHLH转录因子家族成员的分类和进化，分析其在干旱胁迫应激中可能行使的功能，本研究利用多种生物信息学手段从全基因组水平鉴定和解析欧洲山杨bHLH转录因子，并对干旱胁迫下的欧洲山杨根部转录组进行分析.结果显示，欧洲山杨的基因组中有167个bHLH转录因子家族蛋白.通过与拟南芥bHLH蛋白序列进行系统发育分析将其分为15个亚家族，并推测出39个PtbHLH的功能，这些功能表明bHLH转录因子家族涉及到欧洲山杨生长发育的多个生理过程.基因结构和motif分析显示，同一亚家族中几乎所有的PtbHLH都有相似的外显子/内含子排布和蛋白质motif结构.此外，利用RNA-seq数据分析干旱胁迫处理下PtbHLH基因的表达情况，筛选出4个潜在的bHLH抗旱蛋白.

Abstract:

In order to study the classification and evolution of bHLH transcription factor family members in Populus tremula, and predicate their possible functions under drought stress, a variety of bioinformatics methods were used to identify and characterize the bHLH transcription factor in P. tremula at the whole genome level, and analyze the transcriptome of roots tissue under drought stress. The results showed that there were 167 bHLH transcription factor family proteins in P. tremula genome. By phylogenetic analysis with Arabidopsis bHLH protein sequence, it was divided into 15 sub-families, and 39 PtbHLH functions were predicted. These functions indicated that theses bHLHs were involved in multiple physiological processes of the growth and development of P. tremula. Gene structure and motif analysis illustrated that almost all PtbHLHs in the same subfamily have similar exon/intron and motif structure. In addition, RNA-seq data was used to analyze the expression of PtbHLH gene under drought stress, and 4 potential bHLH drought-resistant proteins were screened out.