兴安杜鹃叶抗红色毛癣菌活性成分的筛选

Screening of active components of Rhododendron dauricum L. against Trichophyton rubrum

摘要:

为探究兴安杜鹃叶中对红色毛癣菌起抑制作用的主要活性物质，本研究依据生物活性追踪法，采用多种分离手段从兴安杜鹃叶的乙酸乙酯相中分离鉴定出16个化合物，分别为Daurichromenic acid（1），杜鹃素（2），香荚兰酸（3），二氢槲皮素（4），对羟基苯甲酸（5），伞形花内酯（6），去甲丁香色原酮（7），山楂酸（8），科罗索酸（9），萹蓄苷（10），丁香酸（11），山奈酚（12），槲皮素（13），熊果酸（14），齐墩果酸（15），槲皮素-3-O-β-D呋喃木糖（16），化合物7~9为首次从该植物中分离得到. 抑菌活性测定结果表明，化合物1~3、5~7、11均对红色毛癣菌有抑制活性，其中，化合物2、6、7活性最强，为主要活性物质，其MIC80为0.156 25~0.312 5 mg?mL-1，MBC为1.25~5 mg?mL-1. 本研究首次发现兴安杜鹃叶对红色毛癣菌有较好的抑制活性，并筛选出活性单体.

Abstract:

To explore the main active components that inhibit T.rubrum in the leaves of Rhododendron dauricum L., 16 compounds were isolated and identified from the ethyl acetate phase of Rhododendron dauricum L.’s leaves by a variety of isolation methods based on the bioactivity tracking method.They are identified as Daurichromenic acid(1), farrerol(2), vanillic acid(3), taxifolin(4), 4-Hydroxybenzoic acid(5), umbelliferone(6), 2-Methyl-5,7-dihydroxychromone(7), Maslinic acid(8), corosolic acid(9), avicularin(10), syringic acid(11), kaempferol(12), quercetin(13), ursolic acid(14), oleanolic acid(15),cucurbitacin D(16).Among them,compounds 7~9 were isolated from this plant for the first time.The results of bacteriostatic activity determination showed that compounds 1~3, 5~7 and 11 had certain inhibitory activities against T.rubrum.Among them, compounds 2, 6 and 7 had the highest inhibitory activity and were the main active substances, with MIC of 0.15625~0.3125mg·mL-1 and MFC of 1.25~5 mg·mL-1.In this study, it was first found that leaves of R.dauricum L.had better inhibitory activity against T.rubrum and the active monomer was screened out.