孕激素相关子宫内膜蛋白在胃癌早期诊断、预后中的作用

The role of Progestagen-associated endometrial protein in early diagnosis and prognosis of gastric cancer

摘要:

为使用生物信息学系统分析孕激素相关子宫内膜蛋白(Progestagen-associated endometrial protein, PAEP)在胃癌中的早期诊断和预后预测性能及免疫浸润相关性，该研究构建预测模型ROC曲线和偏差校正曲线检验其性能准确性，并通过细胞实验进一步验证分析结果.生信分析结果显示，与正常组织相比，胃癌组织中PAEP的表达较高，在早期（T1期、N0期、M0期）即有统计学意义（P＜0.001），且诊断胃癌的准确性较高（ROC曲线下面积为0.889）；PAEP高表达的胃癌患者预后较差（P≤0.001）；TNM分期越晚，年龄越大，PAEP表达越高，胃癌患者生存概率越低，偏差校正曲线接近理想曲线（45°线），显示预测结果良好；PAEP的表达与胃癌中的中性粒细胞、巨噬细胞的浸润水平呈正相关趋势，与B细胞、中央记忆型T细胞、T细胞呈显著的负相关趋势.qRT-PCR和Western blot结果显示，HGC-27、BGC-823、MKN-45、SGC-7901和AGS细胞中PAEP基因转录水平和蛋白表达水平均显著高于GES-1. 结果表明了PAEP 蛋白可用于胃癌诊断和预后的生物标志物，并进行了实验验证，其机制与免疫有关.

Abstract:

To use a bioinformatics system to analyze the early diagnosis and prognosis prediction performance and immune infiltration correlation of Progestagenassociated endometrial protein (PAEP) in gastric cancer, this study constructed a predictive model ROC curve and deviation correction curve to test its performance accuracy, and further validate the analysis results through cell experiments. The results of bioinformatics analysis showed that the expression of PAEP in gastric cancer tissues was higher than that in normal tissues, which was statistically significant in the early stages (T1, N0, M0) (P < 0.001), and the diagnostic accuracy of gastric cancer was higher (the area under ROC curve was 0.889). The prognosis of gastric cancer patients with high expression of PAEP was poor (P ≤ 0.001). The later the TNM stage, the older the age, the higher the expression of PAEP, and the lower the survival probability of gastric cancer patients, and the deviation correction curve was close to the ideal curve (45 ° line), indicating a good predictive result. The expression of PAEP was positively correlated with the infiltration level of neutrophils and macrophages in gastric cancer, and negatively correlated with B cells, central memory T cells and T cells. qRT-PCR and Western blot results showed that the transcriptional and protein expression levels of PAEP in HGC-27, BGC-823, MKN-45, SGC-7901 and AGS cells were significantly higher than those in GES-1 cells. The results indicate that PAEP protein can be used as a biomarker in the diagnosis and prognosis of gastric cancer, which is verified by experiments, and its mechanism is related to immunity.