HD2基因调控拟南芥下胚轴发育的功能研究

Functional research of HD2 gene on the regulation of hypocotyl development in Arabidopsis thaliana

摘要:

为探究组蛋白去乙酰化酶HD2基因在拟南芥下胚轴发育中的调控作用，本研究采用野生型（Col-0）、HD2突变体以及过表达植株为材料，研究其在1/2MS以及1/2MS+100 mmol/L甘露醇培养中下胚轴的表型特征，并结合转录组测序数据进一步分析. 实验结果显示，四种过表达株系下胚轴长度较Col-0长，伸长百分比为7.1%～19.5%，差异显著；甘露醇处理后，过表达植株下胚轴较Col-0更长，伸长百分比为14.6%～32.8%，差异更加显著，缩短幅度也更小. hd2a/hd2b和hd2a/hd2c双基因突变体植株在有无甘露醇时，下胚轴长度均显著短于Col-0，但干旱胁迫后变短幅度无明显增加. 转录组数据揭示，HD2基因调控光合系统响应基因影响植株暗形态建成反应，从而影响下胚轴发育. 甘露醇处理后，HD2基因诱导产生非生物刺激响应因子，帮助下胚轴抵抗不良环境. 综上所述，HD2基因在拟南芥下胚轴发育中承担重要调控作用.

Abstract:

To investigate the regulatory role of the HD2 deacetylase gene in Arabidopsis hypocotyls development, this study utilized wild-type plants (Col-0), HD2 mutants and overexpression plants to investigate the phenotypic characteristics of hypocotyl in normal treatment (1/2MS) and drought treatment (1/2MS+100 mmol/L mannitol) and further analyzed them based on transcriptome sequencing data. The experimental results showed that the hypocotyl lengths of the four overexpression plants was longer than that of Col-0 plants, and the elongation percentages ranging from 7.1% to 19.5%, with significant difference. And the hypocotyl lengths of the overexpression plants treated with mannitol were longer than those of Col-0 plants, with an elongation percentages ranging from 14.6% to 32.8%. The difference was more significant, and the shortening amplitude was also smaller. However, the hypocotyls length of doublegenes mutants (hd2a/hd2b and hd2a/hd2c) were significantly shorter than that of Col-0 under both conditions, and the shortening amplitude was not significantly increased after drought stress. Further transcriptome data analysis revealed that the HD2 gene affected hypocotyls development by regulating multiple photoperiod response genes and influencing plant skotomorphogenesis. Moreover, after mannitol treatment, the HD2 gene induced the production of abiotic stimulus response factors, helping the hypocotyls resist adverse environments. In summary, the HD2 gene plays an important regulatory role in Arabidopsis hypocotyl development.