HPLC测定香椿中的有机酸和黄酮等7种活性成分

Determination of Six Flavonoids in Agrimonia Pilosa Ledeb by HPLC

摘要:

摘要：目的 建立超声辅助萃取-高效液相色谱法分离香椿中表儿茶素、没食子酸、儿茶素、芦丁、杨梅素、槲皮素和山奈酚7种活性成分及其含量测定的方法。方法 采用Phenomenex C18色谱柱 (150 mm×4.6 mm, 5 μm)分离7种成分；流动相为甲醇-0.1%醋酸溶液，梯度洗脱；流速：0.8 mL﹒min-1，紫外检测波长：290 nm，柱温：35 ℃。结果 7种成分在15 min内均达到基线分离，线性关系良好（r>0.9991, n=7），平均回收率均在99.23%～104.5% (RSD%<1.26%, n=3)。结论 本实验采用超声波法提取，方法简单、快速、经济、可靠，可用于香椿的质量监控。

Abstract:

Abstract： Objective: The HPLC method is established for separation and determination of epicatechin, gallic acid, catechin, rutin, myricetin, quercetin and kaempferol in Toona sinensis by ultrasonic extraction. Methods: The separation of seven active ingredients is performed on Phenomenex C18 column (150 mm×4.6 mm, 5 μm) with step gradient. Mobile phase is methanol -0.1% acetic acid at the flow rate of 0.8 mL﹒min-1 ; the UV detection wavelength is 290 nm and the coclumn temperature is set at 35℃. Results: The good separation of epicatechin, gallic acid, catechin, rutin, myricetin, quercetin and kaempferol is achieved within 15 min. Calibration curves of the seven active ingrediens show linear relationship (r>0.9991, n=7). The average recoveries are within 99.23%～104.5% (RSD%<1.26%, n=3). Conclusion: This ultrasonic extraction method is simple, fast, economical and reliable.