1,4,7-三氮杂环壬烷合钴与牛血清白蛋白的相互作用

On the interaction of 1,4,7-Triazacyclononan -Co(Ⅱ)with Bovine Serum Albumin

摘要:

利用荧光光谱法研究了1,4,7-三氮杂环壬烷合钴(Ⅱ)与牛血清白蛋白的相互作用，实验结果表明1,4,7-三氮杂环壬烷合钴(Ⅱ)对牛血清白蛋白的荧光猝灭是静态猝灭，并得出在水溶液和胶束溶液中1,4,7-三氮杂环壬烷合钴(Ⅱ)与牛血清白蛋白作用的结合点位数、结合常数和热力学参数，△H>0，△S>0，其分子间的作用力主要表现为疏水作用力。

Abstract:

The interaction of 1,4,7-Triazacyclononan -Co(Ⅱ) (Cyclen-Co(Ⅱ))with bovine serum albumin (BSA) has been investigated by fluorescence method. The results show that the Cyclen-Co(Ⅱ) strongly quenched fluorescence of BSA. It was proved that the fluorescence quenching mechanism belonged to the static quenching by Stern-Volmer equation. According to Lineweaver-Burk equation and thermodynamics equation, the binding constants,binding sates and thermodynamics parameters were obtained at different temperatures in the aqueous solution and in the solutions of Brij35、CTAB、SDS. The hydrophobic interaction forces played a main role in the binding of Cyclen-Co(Ⅱ) with BSA.