双水相萃取红景天苷的研究

Study on Aqueous Two-phase Extraction of Salidroside

摘要:

聚乙二醇（PEG）/盐的水溶液双相体系（ATPS）形成双相行为和双相分配行为的研究.研究了聚乙二醇（PEG）的分子量和质量分数，盐种类，盐的质量分数，离子强度，红景天初始浓度对红景天提取率的影响。并通过正交试验，确定最佳双水相PEG/(NH4)2SO4体系的条件，产生一个简单的预处理方法为提取和测定红景天苷。当双水相体系是20％PEG1000，20％（NH4）2SO4和1%KCl时，提取效果最好，提取率是95.32％。

Abstract:

Based on aqueous two-phase system of polyethylene glycol (PEG)/salt, a simple pretreatment approach was developed for the extraction and determination of salidroside in Rhodiola. The influence factors on phase behavior of forming biphasic and partition behavior of the ATPS was investigated. Studied the factors such as polyethylene glycol (PEG) molecular weight and the mass fraction, the kinds of salt, the salt mass fraction, ionic strength, salidroside initial concentration influence on salidroside extraction rate. Determine the optimum extraction conditions with orthogonal experiment, when the aqueous two-phase system is 20%PEG1000, 20% (NH4)2SO4 and 1% KCl, the extracting effect is best; the extracting rate is 95.32%.Compared with the conventional liquid–liquid extraction or solid-phase extraction, this method was more environmentally, more cost effective.