“三月花”黄花菜的组织培养研究

Study on tissue culture of Hemerocallis citrine Baroni ‘March Flower

摘要:

采用单因素和正交实验方法对“三月花”黄花菜的组织培养技术进行了探索.结果显示:幼叶用0.1 %升汞灭菌12 min时效果最佳,外植体污染率、死亡率均为0, 14 d后外植体启动率为62.5 %.最佳愈伤诱导培养基为MS + 2 mg/L 2,4-D + 0.1 mg/L 6BA,外植体出愈率为86.67 %;愈伤组织最适分化培养基为MS + 2 mg/L 6-BA + 0.5 mg/L IBA,愈伤组织分化率达到80 %,平均每块愈伤组织不定芽个数为1.72.最佳生根培养基为1/2 MS+0.5 mg/L NAA, 生根率达到 93.33 %,平均每个芽苗生根数为5.83条,试管苗炼苗移栽后,成活率达 95 %.利用该组织培养技术,幼叶外植体通过愈伤组织途径获得“三月花”黄花菜再生苗.

Abstract:

Single factor and orthogonal experiment methods were used to explore the tissue culture technique of Hemerocallis citrine ‘March Flower’. The results showed that the best effect was when the young leaves were treated with 0.1 % mercuric chloride for 12 min with no pollution and death and the activation rate was 62.5 % after 14 days. MS + 2 mg/L 2,4-D + 0.1 mg/L 6-BA was the optimal induction medium with the 86.67 % recovery rate, MS + 2 mg/L 6-BA + 0.5 mg/L IBA was the best differentiation medium with the 80 % differentiation rate, and the average number of adventitious buds per block was 1.72. 1/2 MS + 0.5 mg/L NAA was the best rooting medium, the rooting rate was 93.33 %, the average number of rooting per shoot was 5.83. The survival rate of transplanted test tube seedling was 95 %. Using the tissue culture technique, the young leaf explants obtained the "March flower" daylily regeneration seed through the callus pathway.